全文获取类型
收费全文 | 1234篇 |
免费 | 70篇 |
国内免费 | 63篇 |
出版年
2023年 | 7篇 |
2022年 | 12篇 |
2021年 | 20篇 |
2020年 | 25篇 |
2019年 | 31篇 |
2018年 | 33篇 |
2017年 | 24篇 |
2016年 | 28篇 |
2015年 | 31篇 |
2014年 | 35篇 |
2013年 | 72篇 |
2012年 | 35篇 |
2011年 | 43篇 |
2010年 | 29篇 |
2009年 | 39篇 |
2008年 | 52篇 |
2007年 | 46篇 |
2006年 | 39篇 |
2005年 | 42篇 |
2004年 | 30篇 |
2003年 | 31篇 |
2002年 | 20篇 |
2001年 | 32篇 |
2000年 | 22篇 |
1999年 | 19篇 |
1998年 | 11篇 |
1997年 | 13篇 |
1996年 | 24篇 |
1995年 | 18篇 |
1994年 | 17篇 |
1993年 | 22篇 |
1992年 | 19篇 |
1991年 | 11篇 |
1990年 | 5篇 |
1989年 | 3篇 |
1988年 | 4篇 |
1987年 | 4篇 |
1986年 | 4篇 |
1985年 | 15篇 |
1984年 | 54篇 |
1983年 | 45篇 |
1982年 | 58篇 |
1981年 | 65篇 |
1980年 | 53篇 |
1979年 | 71篇 |
1978年 | 36篇 |
1976年 | 3篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1973年 | 9篇 |
排序方式: 共有1367条查询结果,搜索用时 531 毫秒
1.
Bioassays were used to study the infectivity of cytoplasmic polyhedrosis viruses (CPVs), nuclear polyhedrosis viruses (NPVs) and entomopoxviruses (EPVs) contained in 16 nests of the paper waspPolistes hebraues F. in Réunion Island. Several virosis were propagated from 6 nest contents in 5 Lepidoptera:Catopsilia thauruma Saalmüller,Catopsilia florella F.,Callixena versicolora Mabille,Polydesma umbricola Boisduval andEagris sabadius Boisduval. Each of the previous species supported the development of one or two virosis and therefore, wasp nests must be considered as accumulating centres where infectivity of occluded entomoviruses is preserved. The accurate origin of the virosis propagated through bioassays was searched for using ecological investigations on similar natural diseases, REN analysis and a cross-transmission test onSpodoptera mauritia Boisduval. The nuclear polyhedrosis and one cytoplasmic polyhedrosis appearing in bioassays can be assigned respectively to viruses produced byC. thauruma andC. versicolora; the other virosis must be considered as developing in alternate or substitution hosts. Wasp nests could therefore be used to detect the presence of specific viruses in an environment and to collect new virus isolates. 相似文献
2.
3.
S C Pan 《Experimental parasitology》1978,45(2):274-286
Established cultures of human skin-muscle cells were used for determining the parasite—host cell relationship of Trypanosoma cruzi amastigotes (12–16 passages) cultured in a cell-free medium (F-69) at 37 C. The medium used for this experiment was tissue culture fluid M-199 enriched with 10% fetal bovine serum and relatively high concentrations of ATP, ADP and AMP. Amastigotes entered skin-muscle cells incubated at 32 or 35 C, multiplied and completed their intracellular life cycle in about 7 days. At 35 C, 23.6% of cells became infected in 7 days and at 32 C, 43.6% were infected in 5 days. The higher infection rate of cultured cells at 32 C was probably due to more frequent and prolonged cell-parasite contact, as amastigotes multiplied in the tissue culture medium and remained viable for a longer period at the lower temperature. As a control, epimastigotes were used to infect skinmuscle cells. Epimastigotes transformed into metacyclic trypomastigotes before entering host cells, multiplied, and completed the intracellular life cycle. We conclude that the amastigotes cultured in F-69 at 37 C are biologically similar to intracellular amastigotes from the vertebrate host, in that both can multiply and complete the life cycle intracellulary. 相似文献
4.
Adaptative significance of a temperature induced diapause in a cosmopolitan parasitoid of Drosophila
Abstract. 1. We have previously demonstrated pre-pupal diapause in a tropical population of Leptopilima boulardi Barbotin, Carton and Kelner-Pillault (Cynipidae, Hymenoptera), a larval parasitoid of Drosophila melanogaster Meigen. Prepupal diapause was induced by low temperature (17.5°C) but absent at 25°C; it was independent of photoperiod.
2. Four populations which originated from different latitudes (4°S to 44°N) exhibit the same response to temperature without any major genetic geographical variation.
3. In temperate areas, this diapause may permit a phenological and temporal coincidence between the parasitoid and its host whose larval growth is only possible for temperatures above 13°C.
4. The significance of the persistence of this diapause in tropical areas is discussed. 相似文献
2. Four populations which originated from different latitudes (4°S to 44°N) exhibit the same response to temperature without any major genetic geographical variation.
3. In temperate areas, this diapause may permit a phenological and temporal coincidence between the parasitoid and its host whose larval growth is only possible for temperatures above 13°C.
4. The significance of the persistence of this diapause in tropical areas is discussed. 相似文献
5.
A feature of many sugarcane fields is the patchy growth. This is often thought to be due to physical or chemical differences in the soil. In this paper we investigate the causes of growth heterogeneity of sugarcane on a sandy soil in KwaZulu-Natal, South Africa. To identify the factors that were associated with the good and poor areas, soil texture, pH, organic matter content and a number of soil chemical elements and nematode community data were subjected to principal component analysis (PCA). The numbers of each of the nematode species (Meloidogyne sp., Pratylenchus zeae, Helicotylenchus dihystera, Xiphinema elongatum and Paratrichodorus sp.) were first converted to relative proportions of ectoparasites and endoparasites. The data were collected from the 2nd ratoon crop of a nematicide trial, where half of the plots had been treated with aldicarb in the preceding two crops. Yields of control plots varied from 34.7 to 126.8 t cane ha–1 and from 85.4 to 138.7 for the treated plots. The yield data were centred and normalised separately for the treated and control plots and the values projected on the trial map to study spatial distribution. Plots with above-average yields, whether treated or untreated, occurred in the lower part of the trial site. The PCA factorial values were also projected onto the map of the trial. According to the first factor of the analysis of the abiotic soil characteristics in the 0–20 cm surface layer, the trial site could be divided into two areas, one on the left and one on the right. PCA of the soil data from the 0–20 and 20–40 cm layers showed that there was little or no difference between the two that might explain the two growth areas. However, analysis of the nematode community distinguished two main areas that largely corresponded to the distribution of the plots of low and high yielding cane. Correlation analysis confirmed the relationship between nematodes and yield. H. dihystera was positively correlated with yield of cane whereas the reverse was true for the Meloidogyne species. 相似文献
6.
7.
The parasitic wasp Cephalonomia tarsalis parasitizes larvae of the saw-toothed grain beetle Oryzaephilus surinamensis, which feed on wheat grains. In contrast to most other host–parasitoid systems studied so far, the grain beetles are highly mobile within their habitat, bulk of grains in grain stores. This should increase the wasps’ problem to locate the hosts. To study the host-finding strategy of C. tarsalis females, the reaction of wasps to different grain and host-derived odour sources was tested in a four-chamber-olfactometer. These experiments revealed that wasps were attracted by healthy grains and mechanically damaged grains. In direct comparison, healthy and mechanically damaged grains are equally attractive. Both potential sources of host-derived odours, host faeces and trail-traces of larvae on filter paper were attractive to the wasps. The response to trail-traces vanished 30 min after larvae had been removed from the filter paper. With respect to the specificity of the odours, it turned out that wasps were attracted to odours from the seed–host complexes from O. surinamensis and Oryzaephilus mercator and the non-host complex of larvae of the granary weevil Sitophilus granarius in wheat grains. Odours from the seed–host complex were preferred. From these results, we hypothesize that host habitat location in C. tarsalis is achieved by using grain-derived odours. Within the habitat, wasps search for kairomones from host faeces and host trails. Following these larval trails finally leads wasps to their hosts. 相似文献
8.
Jan Sielewiesiuk 《Acta Physiologiae Plantarum》2002,24(4):399-406
An explanation of photodamages to PS II at low light intensities is proposed. The explanation is based on the two following
postulates: 1. Intermediate states of the water-oxidizing complex can undergo parasitic reduction to lower degrees of oxidation.
2. Photosynthetic apparatus can minimize the unfavorable effect of such reduction by changes in the number of active reaction
centers of PS II. Some steady-state characteristics were calculated under these assumptions. The results obtained show that
the frequency of P680 excitations at low light intensities becomes as high as that at saturating light provided that the rate
of parasitic reduction is comparable with the rate of the slowest reaction in the cycle of the water- oxidizing complex conversions. 相似文献
9.
R. D. Harrison 《Population Ecology》2001,43(1):63-75
Borneo has a perhumid climate but occasional severe droughts have an important impact. Droughts may affect the composition and size structure of plant communities through differential mortality or, via their impact on the availability of plant resources, affect plant–animal interactions. From January to April 1998, northern Borneo suffered a very severe drought linked to the El Niño Southern Oscillation event of 1997–1998. In this article, the impacts of this drought on the rain forest at Lambir Hills National Park, Sarawak, are considered with special reference to a keystone plant group, the figs. Small fires entered the edge of the forest from the roadside, killing saplings, climbers, and understory trees. Community-wide mortality for adult trees was 0–7 times higher than in nondrought years, with larger trees showing a greater proportional increase. In figs, mortality was significantly higher in pioneers, but hemiepiphytes and roadside species were unaffected. Phenology was substantially affected. Leaf and flower/fruit production decreased or ceased during the drought and increased suddenly following renewed rain. Pollinators of dioecious figs became locally extinct during the drought, and other plant–animal interactions may also have been disrupted. The frequency and severity of droughts has increased substantially in the past three decades, and climate models suggest this may be the result of global warming. The impacts of the 1998 drought at Lambir Hills National Park suggest that, should this trend continue, a substantial alteration of habitats and overall loss of biodiversity can be expected in Borneo. 相似文献
10.
K. G. Davey 《Journal of nematology》1995,27(4):433-440
While nematodes are sometimes regarded as osmoconformers, at least one species is capable of short-term osmoregulation over a wide range of osmotic environments, and the principal site of osmoregulation is the body wall. This general osmoregulation is important to the life of the nematode not only in confronting variations in the environment, but also in maintaining its hydrostatic skeleton. There is also evidence suggesting that compartments exist in some nematodes and that water exchange between the compartments is limited and slow. The ability to regulate the internal movements of water is important in molting and in the infective process. Hormones may be the mediators of osmotic control. 相似文献